Journal: Current Issues in Molecular Biology
Article Title: The Enhancement of Regulatory T Cell Maturation and Th1/Th2 Balance through FOXP3 Expression by Lactobacillus paracasei in an Ovalbumin-Induced Allergic Skin Animal Model
doi: 10.3390/cimb46100636
Figure Lengend Snippet: Effects of Lactobacillus paracasei subsp. paracasei NTU 101 on the cell proportions of ( A ) dead splenocytes, ( B ) CD4+/IFN-γ+, ( C ) CD4+/IL-4+, ( D ) CD4+/IL-17+, and ( E ) CD4+/CD25+/FOXP3+ in splenocytes from EC-sensitized mice. Each value is expressed as mean ± SD ( n = 8). Statistical comparison of differences between each group: * denotes p -value less than 0.05 ( p < 0.05); ** denotes p -value less than 0.01 ( p < 0.01); *** denotes p -value less than 0.001 ( p < 0.001). The NOR group (control) received saline and a standard diet. The OVA group underwent sensitization and skin irritation with ovalbumin (OVA) on a normal diet. The CM group had the same OVA treatment plus prednisolone. The NTU 101 group received OVA treatment and NTU 101 at 1 × 10 11 CFU/day. The LGG group underwent OVA treatment and received L. rhamnosus GG ATCC 53103 at 1 × 10 11 CFU/day.
Article Snippet: Cell staining was performed using a panel of monoclonal antibodies: FITC-labeled anti-mouse CD4 (clone: RM4-5, eBioscience, San Diego, CA, USA); PE-labeled rat anti-mouse CD25 (clone: PC61.5, eBioscience); PerCP-Cy™ 5.5-labeled rat anti-mouse IFN-γ (clone: XMG1.2, BD, NJ, USA); PerCP-Cy™-labeled rat anti-mouse IL-4 (clone: 11B11, BioLegend, San Diego, CA, USA); PerCP-Cy™ 5.5-labeled rat anti-mouse FOXP3 (clone: FJK-16s, Invitrogen, Carlsbad, CA, USA); and PerCP-Cy™ 5.5-labeled rat anti-mouse/rat IL-17A (clone: eBio17B7, eBioscience).
Techniques: Comparison, Control, Saline